Background and Objective: The second victim (SV) phenomenon concerns healthcare workers (HCWs) whose involvement in a medical error, as well as non-error patient safety events or near-miss events, has affected their well-being. Its prevalence ranges from 10% to 75%, and can predispose HCWs to burnout and depression, increasing the probability of committing errors. The primary aim of our study was to determine the prevalence of HCWs involved in an adverse patient safety event in Friuli Venezia Giulia Region (Italy). The secondary aims were to use latent profile analysis to identify profiles of SVs and factors influencing profile membership, and to evaluate the relationship between the severity of symptoms and desired support options. Methods: A cross-sectional survey through the IT-SVEST tool was conducted in five Local Health Authorities, and all HCWs involved in direct patient care were invited. Descriptive statistics were conducted for all variables and correlations were evaluated with Kendalls tau-b test. Latent profile analysis was based on the scores of dimensions measuring SVs symptoms. Factors affecting profile membership were assessed through multinomial logistic regression. Results: A total of 733 HCWs participated. Of them, 305 experienced at least one adverse event (41.6%). Among dimensions measuring SVs symptoms, psychological distress had the highest percentage of agreement (30.2%). Three latent profiles were identified: mild (58.7%), moderate (24.3%), and severe (17.0%) symptoms. Compared to the mild symptom profile, the severe symptoms profile was positively associated with the agreement for extra-occupational support and negatively associated with the agreement for organizational support. The severity of symptoms was directly associated with the desire for support strategies. Conclusions: The prevalence of HCWs involved in adverse events is consistent with the literature. Three latent profiles have been identified according to SVs symptoms and the higher the severity of symptoms, the greater the reliance on extra-occupational support.