Background and Objective: Malaria is a major public health problem in Sub-Saharan Africa including Côte d’Ivoire. It is a deadly disease for children under five year’s old and pregnant women. The prevalence of malaria is not well studied in oil palm plantations in Aboisso district. Therefore, the aim of this study is to quantify the burden of Plasmodium malaria infection in two oil palm plantations in South Easten region of Côte d’Ivoire. Methods: Two cross-sectional surveys bearing on 388 children aged 1 to 14 years old from two oil palm agro systems namely Ehania-V1 and Ehania-V5. During the dry and the peak of the long-wet seasons, finger pricked blood was used to prepare thick and thin blood smears. Results: 66.2% of children were identified as afebrile, with axillary temperature below 37.5°C. According to villages, this was 63.7% in Ehania-V1 and 61.4% in Ehania-V5. Microscopy identified an overall Plasmodium falciparum infection prevalence of 35.3%. 19.1% of children in Ehania-V1 and 53.3% in Ehania-V5 harbour Plasmodium falciparum the dominant parasites. Plasmodium falciparum was not associated with gender, age-group, fever and mosquito net use (P>0.05). The prevalence of Plasmodium in febrile (43.7%) and afebrile (59.3%) was not similar in children of Ehania-V5 (p=0.04). Children from Ehania-V5 were significantly more infected in the wet (62.7%) season than the dry (41.5%) (p=0.004). Significant difference were observed in Plasmodium falciparum between the two villages during both the dry (p=0.005) and wet season.