Background: The World Health Organization Framework Convention on Tobacco Control (FCTC) includes measures to reduce demand for and supply of tobacco. The measures related to demand reduction include raising taxes, providing cessation services, promoting smokefree public places, banning advertising, and raising awareness. However, there are a limited number of measures for supply reduction, and these mainly include fighting illicit trade, banning sales to minors and providing alternatives to tobacco workers and growers. This scoping review Aims to identify additional potential measures of retail environment regulations to reducing tobacco supply and consequently reducing tobacco use. Methods: This scoping review examines interventions, policies, and legislation to regulate the tobacco retail environment to reduce tobacco availability. FCTC and its Conference of Parties decisions, grey literature including tobacco control databases and the databases PubMed, EMBASE, Cochrane Library, Global Health, and Web of Science were systematically searched, complemented by a scoping communication with the focal points of the 182 FCTC Parties. Results: Themes of potential measures to reduce tobacco availability by regulating retail environment were identified among both FCTC and non-FCTC measures. Studies show the effects of regulation of the retail environment in influencing overall tobacco purchases, and there is strong evidence that having fewer retail outlets reduces the level of impulse purchasing of cigarettes and tobacco goods. The measures covered by FCTC are more implemented than ones not covered by it. Conclusions: Further studies to assess effectiveness and implementation of such measures, and the adoption of the effective ones to be covered under FCTC decisions would probably increase their adoption globally and may reduce tobacco availability.