Background: Mental health, therefore, is an integral part of human health and well-being. Mental illness constitutes four of the ten most common causes of the worldwide burden of disease, yet it remains low on the agenda of policymakers, particularly in developing countries like Bangladesh. This study aimed to assess the perception of public health professionals regarding mental health and illness. Methodology: This study was a cross-sectional study using a quantitative approach and a non-probability convenient sampling technique. Both males and females having at least six months of total working experience in the public health sector were the inclusion criteria. Perceived Stigma Questionnaire (PSQ) scale was used to measure level of perception and stigma. Results: The majority of the respondent reported peace of mind is called mental health. They also expressed there is no health without mental health. Few of them have no idea about this, they just read books and papers about it, but they don’t have any practical knowledge regarding mental health. Among all the respondents, 16.7% respondents had some training, and 52.1% respondents had no education on mental health. The majority (68.8%) strongly agreed that mental illness could happen to anybody. On the other hand, around 33% of respondents stated females are more likely to have a mental illness than males, whereas 18% of respondents disagreed with this statement. Conclusions: Addressing stigma is one of the directions that need timportance to improve mental health and mental health care mainly because the rejecting behaviour of others may bring more significant disadvantage to the afflicted individuals than the mental condition itself. There is a paucity of studies on this particular research area, and it is believed that even this small-scale study can point toward a direction for future wide-scale investigations and intervention research in mental health.