Background and Objective: This study targeted public health nurses (PHNs) who provide health support in lifestyle-related diseases as public servants of their local government in Japan, and investigated the influence of having or not having experience in providing health support to former prisoners on their perceived importance of collecting information during interviews. Methods: A cross-sectional study was conducted using self-administered questionnaire. Three copies of the questionnaire were mailed to each of the 1,724 municipalities of Japan (5,172 copies in total) from November 5 to December 20, 2021. Responses were obtained on a four-point scale of "very important", "important", "not very important" and "not important" for seven types of information that should be ascertained when interviewing former prisoners. This survey was conducted after review and approval by the Ethics Committee of the presenters institution. Results: A total of 1,371 responses were received (response rate: 26.5%), with 1,324 valid responses. Of the respondents, 204(15.4%)of PHNs indicated that they had experience in providing health support to former prisoners. Logistic regression analysis was conducted using the experience of providing support to former prisoners as the dependent variable, with years of experience and gender as adjustment variables, in relation to the perceived importance of information to be confirmed when meeting with former prisoners. The information that PHNs with experience providing support to former prisoners perceived as "most important" for health support were family structure (AOR:1.52, 95%CI: 1.09-2.10), economic status (AOR:1.64, 95%CI: 1.20-2.25), contact information of supporters (AOR:2.03, 95%CI: 1.48-2.79) and type of crimes (AOR:1.55, 95%CI: 1.10-2.20), while length of prison sentence (AOR:0.96, 95%CI: 0.64-1.45), medical history (AOR:0.97, 95%CI: 0.61-1.53) and educational status (AOR:1.17, 95%CI: 0.85-1.61) were not statistically significant. Conclusions: When providing health support to former prisoners, PHNs more likely addressed type of crimes committed rather than their length of prison sentence.