Background and Objectives: Dengue fever is a mosquito-borne viral disease that causes nearly 390 million dengue infections annually, particularly in the tropical and subtropical regions of the world. According to the World Health Organization (WHO), 40 % of the world’s population lives in these areas that are classified to be at high risk for Dengue infections. Sudan is one of the countries with a continuous risk of developing dengue fever outbreaks with 1197 reported cases in 2019. Our study aimed to assess the knowledge, attitude, and practice patterns regarding dengue fever among the public living in Khartoum state of Sudan. Methods: A descriptive cross-sectional community-based survey was conducted in August 2021, among 532 participants. Data were collected using a pretested, structured online questionnaire designed on Google form and were analyzed using Statistical Package for Social Science (SPSS). Results: Among the survey respondents (n=532), (56%) were male, (36.1%) aged 26-35, (75%) were in universities and institutes, (49.2%) were married and (60%) were working. (7%) had a personal history of dengue infection, while (19.7%) had reported knowing someone who get infected. Social media was the main source of information. Knowledge scores were significantly associated with occupation, marital status, source of information regarding dengue fever, history of personal infection, and family or friends’ previous infection (P<0.05). The overall correct rate of the knowledge questionnaire was (52.12%). (69.7%) agreed that dengue fever is a serious illness in Sudan. (94%) used fans and (93.4%) covered their water containers. Conclusions: This study revealed that participants had an intermediate level of knowledge, while most participants showed a positive attitude and appropriate practices. The study highlighted that people with a personal or family history of infection had relatively higher knowledge compared to other groups. This warrants the development of appropriate public health interventions and educational programs.