Intersectoriality as an instrument of public health policy provides efficiency, effectiveness, and efficacy of actions, enabling the resolution of complex problems. This study describes the mapping of the intersectoral agendas of the MOH of Brazil between September and December 2022. It was carried out an exploratory descriptive situational diagnosis with a quali-quantitative approach to raise and characterize intersectoral action involving MOH. The research developed bibliographic surveys regarding the intersectoriality, strategic concepts about health policies/programs, and main normative acts that guide the intersectoral action. The data was collected using a standard instrument together with the technical areas and document analysis. It registered the participation of 100% of the technical areas requested, namely 398 in collegiate bodies and 116 in intersectoral instances. The main findings consist of confirmation the strong relationship between the health sector and Social Assistance, and citizenship; Human rights; Science and technology; Justice and public safety; Environment; Work and income; and Education, as an expression of the social determination of the health-disease process. Despite a polysemic understanding of the concept of intersectoriality, it can be inferred that this is a reality in terms of MOH actions, being presented as a guideline in the policies and programs mapped. However, it is a challenge, as the intersectoral organization demands the combination of different and complex knowledge, powers, practices, goals, resources, and joint monitoring. It is suggested the institutionalization of mapping as well as its recognition as an instrument that promote knowledge, provide transparency to the universe of intersectoral public policies, provides social collaboration and control, synergy between internal and external actions, minimizes overlaps, and enhances the performance of the MoH concerning other sectors. It attempts to pursue synergy between public policies to improve the populations health and the performance of the Unified Health System (SUS).