In the South, there were top 5 provinces with high accidents and injuries, including Satun, Trang, Songkhla, Phatthalung and Surat Thani provinces, respectively, with the highest rate of injury at 17.37 per thousand. Preventive actions, promoting safety, occupational health and working environment are therefore important in order to foster worker health and safety working environment. The main concept of Integrated Total Worker Health(iTWH) is aspects linked as an integrated operation, promotion and prevention work-related safety and health hazards, reducing injuries and illnesses for better workers well-being. iTWHs focus is on the safety and good health of workers. However, little research has examined the impact of iTWH interventions in Southern enterprises. The aim of this study was to explore and characterize health and safety practices, working conditions, occupational health and safety policies, and programs in Southern enterprises from an iTWH perspectives(n=6). Engaging science studies and approaches, site visits, walk-through surveys, questionnaires interviews (n=660), group discussion were conducted with enterprises, between 150 and 1,250 employees in 2022. Qualitative data from 54 interviews and tracking documents were coded and thematically analyzed. By assessing the context of the enterprise settings in which the intervention was situated, we explored factors preventing and facilitating the working conditions and improvement, including organizational factors and atmosphere, engaging employees, low-cost strategies, evaluation, and integration. The enterprises have fewer resources and financial supports for occupational health and safety, are more likely iTWH precarious, and have higher rates of occupational injury and illness compared with larger enterprises. Interventions that address the promotion of health and well-being in addition to conventional occupational intervention and health hazards are crucial supports for improving iTWH. These themes overlapped with both the different governmental agencies and fundamentals. As iTWH expands to organizations of all sizes, it is necessary to address the needs of the enterprises.