Gender-sensitive interventions for alcohol prevention among adolescents often lead to binary tailoring for girls and boys. Increased societal and legal recognition of sexual and gender minorities and participatory research with the target group demand a broader understanding of gender. As a promising educational tool, virtual reality offers opportunities for tailoring interventions, e.g. by using gender avatars and corresponding scenarios. Therefore, the present study explores LGBTQIA+ adolescents perceptions of gender tailoring and portrayal in the digital application Virtual LimitLab–a virtual reality-simulation to train refusal-skills towards peer pressure regarding alcohol consumption. The study aims at investigating how digital interventions should be further developed to include sexual and gender diversity. Qualitative interviews with 16 LGBTQIA+ adolescents in Berlin, Germany were conducted after individual simulation testing. The collected data were analysed using Braun and Clarke’s thematic analysis with a reflexive orientation. Four themes were identified: statements on the relevance of gender and opinions on tailoring options, flirt orientation, and the other characters within the simulation. Deviating positions were found on the relevance of gender and different tailoring options by avatars to include queer users. Consensus was expressed on the wish for more representation of diversity among the characters, not only regarding sexual orientation and gender identity, but also regarding e.g. the inclusion of racialised peers among the main actors. Furthermore, the expansion of flirting options in the simulation by adding bisexual and asexual/aromantic options besides same-gender options was suggested. In the case of the short virtual simulation Virtual LimitLab, no queer avatar is needed, but better representation of diversity among the characters and a more complex understanding of gender that goes beyond binarity and reflects diversity in its intersectionality.