Background: The covid-19 pandemic exacerbated inequities in healthcare for vulnerable populations and exposed structural gaps in the health system in India. During the pandemics peak, access to quick, accurate, and affordable testing method was scarce due to overburdened public and private testing centers. Self-testing kits for home-based use for detecting the sars-cov-2 virus were introduced as a tool to bridge the gaps of conventional testing. This study aimed to determine the usability of SARS-COV-2 self-tests in a peer-assisted model among factory workers in Bengaluru, India. Methods: This mixed-method cross-sectional study of 106 factory workers in Bengaluru, India evaluated the usability of a nasal sampling-based covid-19 antigen self-test kit (PANBIO™) in a peer-assisted model from February to march 2022. An observer used a product-specific usability checklist consisting of critical and non-critical steps in the IFU to record the usability of the test kit and the mobile application as the participant performed the test with peer assistance. Results: the average usability score of the test kit was 80.69% and the average usability score for uploading results through the mobile application was 34.01%. 82.07% of the participants correctly interpreted pictures of positive results with 62.0% of participants being confident in performing and interpreting the test with the assistance of a peer. Whereas, 43.9% of participants were confident in performing and interpreting the test on their own suggesting that peer assistance is a key determinant for the usability of self-tests in marginalized populations. Conclusions: Results of the usability and acceptability study suggest that a nasal sampling-based self-testing kit for covid-19 shows overall moderate to high levels of usability and high levels of acceptability in a peer-assisted workplace testing model. Determining the feasibility of implementing this model at scale is the next step to inform and advocate for other workplace self-testing programs for vulnerable populations.