Background and Objective: Cancer-related pain/discomfort might be a potential cause of persistent symptoms that have negative impacts or alter patients' quality of life (QOL). The aim of this study was to assess the pain/discomfort among cancer patients undergoing chemotherapy in Bangladesh. Methods: A hospital-based cross-sectional study was conducted on 415 cancer patients using a convenient sampling technique. Adult cancer patients' socioeconomic and self-reported EuroQol 5-Dimension 5-Level (EQ-5D-5L) data were collected between January 2022 and April 2022. Frequency and percentages were used to represent descriptive analysis, and Chi-Square test was performed to assess possible influencing factors with pain/discomfort. Results: Among our respondent participants, 258 (68%) patients were in the age groups of 30-59 years, and most of them 272 (66%) were males. The majority 113 (27%) of the subjects was lung cancer, 169 (41%) had stage IV cancer, and 175 (42%) completed 3-4 chemotherapy cycles. In total, 131 (32%), 141 (34%), 85 (20%), and 47 (11%) patients reported slight, moderate, severe, and extreme pain/discomfort, respectively. The results showed significant associations of pain/discomfort with cancer stage (p<0.001), smokeless tobacco use (p<0.001), chemo cycle number (p=0.001), and work status (p=0.001). Conclusions: The majority of patients experienced pain or discomfort, which was associated with cancer stages, smokeless tobacco use, chemotherapy cycle number, and work status, resulting in a poor quality of life. We need to take the initiative in pain/discomfort management or health education interventions to reduce cancer survivors' pain or discomfort, which might enhance cancer survivors' QoL in the future.